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Mean-normalised concentration data for CC (blue) and LC (red). All variants are considered together for this analysis. Significant differences are present between the cohort medians for IgG1, IgG3 and IgG4 using a Mann Whitney U test with Holm-Sidak correction (Data shown in ). (*) indicates significance p<0.05, (**) indicates significance p<0.005. Some outliers for the IgG1 control data lie outside of the plotted y-values and can be found in

Journal: medRxiv

Article Title: Mass-Standardised IgG Response to Fourteen SARS-CoV-2 Spike Protein variants and Antibody Subclass analysis for IgG subclasses and IgE for a Long COVID Patient Cohort

doi: 10.64898/2026.01.26.26344863

Figure Lengend Snippet: Mean-normalised concentration data for CC (blue) and LC (red). All variants are considered together for this analysis. Significant differences are present between the cohort medians for IgG1, IgG3 and IgG4 using a Mann Whitney U test with Holm-Sidak correction (Data shown in ). (*) indicates significance p<0.05, (**) indicates significance p<0.005. Some outliers for the IgG1 control data lie outside of the plotted y-values and can be found in

Article Snippet: IgG1 spike reference materials from Sinobiological (40590-D001) were used to calibrate the surface density of spike protein for each variant, binding to the conserved S2 region.

Techniques: Concentration Assay, MANN-WHITNEY, Control

Single patient results showing the variant specific response to each IgG subclass measured separately for IgG1-IgE but for all variants simultaneously: A) Control sample and B) Long Covid sample: IgG Subclass concentrations IgG1-4: coloured as pink (IgG1), blue (IgG2), yellow (IgG3), green (IgG4) and grey (IgE).

Journal: medRxiv

Article Title: Mass-Standardised IgG Response to Fourteen SARS-CoV-2 Spike Protein variants and Antibody Subclass analysis for IgG subclasses and IgE for a Long COVID Patient Cohort

doi: 10.64898/2026.01.26.26344863

Figure Lengend Snippet: Single patient results showing the variant specific response to each IgG subclass measured separately for IgG1-IgE but for all variants simultaneously: A) Control sample and B) Long Covid sample: IgG Subclass concentrations IgG1-4: coloured as pink (IgG1), blue (IgG2), yellow (IgG3), green (IgG4) and grey (IgE).

Article Snippet: IgG1 spike reference materials from Sinobiological (40590-D001) were used to calibrate the surface density of spike protein for each variant, binding to the conserved S2 region.

Techniques: Variant Assay, Control

Antibody IgG subclass and IgE concentration distributions presented by SARS-CoV-2 Spike protein variant: CC (blue) and LC (red) cohorts. (A) IgG1; (B) IgG2, (C) IgG3, (D) IgG4 and (E) IgE. The boxplots show the medians and interquartile range. The vertical lines on each box point to the extremes of the distributions. Significant results to the p< 0.05 (*) and p<0.005 (**) level determined by the Mann-Whitney U test with Holm-Sidak correction applied

Journal: medRxiv

Article Title: Mass-Standardised IgG Response to Fourteen SARS-CoV-2 Spike Protein variants and Antibody Subclass analysis for IgG subclasses and IgE for a Long COVID Patient Cohort

doi: 10.64898/2026.01.26.26344863

Figure Lengend Snippet: Antibody IgG subclass and IgE concentration distributions presented by SARS-CoV-2 Spike protein variant: CC (blue) and LC (red) cohorts. (A) IgG1; (B) IgG2, (C) IgG3, (D) IgG4 and (E) IgE. The boxplots show the medians and interquartile range. The vertical lines on each box point to the extremes of the distributions. Significant results to the p< 0.05 (*) and p<0.005 (**) level determined by the Mann-Whitney U test with Holm-Sidak correction applied

Article Snippet: IgG1 spike reference materials from Sinobiological (40590-D001) were used to calibrate the surface density of spike protein for each variant, binding to the conserved S2 region.

Techniques: Concentration Assay, Variant Assay, MANN-WHITNEY

Correlation between Total IgG and subclass measurements: A) Control Cohort, B) Long Covid Cohort. Marker shape denotes subclass: IgG1 (o), IgG2 (x), IgG3 (□), IgG4 (◊) and IgE (Δ). Regression lines are ordered vertically and IgG1 and IgG4 production dominates in long COVID patients compared with IgG1 and IgG2 in controls; A) controls IgG1-IgG2-IgG3-IgG4-IgE; and B) long COVID IgG1-IgG4-IgG2-IgE-IgG3.

Journal: medRxiv

Article Title: Mass-Standardised IgG Response to Fourteen SARS-CoV-2 Spike Protein variants and Antibody Subclass analysis for IgG subclasses and IgE for a Long COVID Patient Cohort

doi: 10.64898/2026.01.26.26344863

Figure Lengend Snippet: Correlation between Total IgG and subclass measurements: A) Control Cohort, B) Long Covid Cohort. Marker shape denotes subclass: IgG1 (o), IgG2 (x), IgG3 (□), IgG4 (◊) and IgE (Δ). Regression lines are ordered vertically and IgG1 and IgG4 production dominates in long COVID patients compared with IgG1 and IgG2 in controls; A) controls IgG1-IgG2-IgG3-IgG4-IgE; and B) long COVID IgG1-IgG4-IgG2-IgE-IgG3.

Article Snippet: IgG1 spike reference materials from Sinobiological (40590-D001) were used to calibrate the surface density of spike protein for each variant, binding to the conserved S2 region.

Techniques: Control, Marker

Journal: medRxiv

Article Title: Mass-Standardised IgG Response to Fourteen SARS-CoV-2 Spike Protein variants and Antibody Subclass analysis for IgG subclasses and IgE for a Long COVID Patient Cohort

doi: 10.64898/2026.01.26.26344863

Figure Lengend Snippet:

Article Snippet: IgG1 spike reference materials from Sinobiological (40590-D001) were used to calibrate the surface density of spike protein for each variant, binding to the conserved S2 region.

Techniques: Transformation Assay

The variant profiles showing the long COVID classification triggers: (A) IgG1 below threshold, T; and (B) IgG3 (C) IgG4 (D) IgE above threshold, T, derived from the percentile (T) in the mean-normalised variant concentration distributions in the Control Cohort.

Journal: medRxiv

Article Title: Mass-Standardised IgG Response to Fourteen SARS-CoV-2 Spike Protein variants and Antibody Subclass analysis for IgG subclasses and IgE for a Long COVID Patient Cohort

doi: 10.64898/2026.01.26.26344863

Figure Lengend Snippet: The variant profiles showing the long COVID classification triggers: (A) IgG1 below threshold, T; and (B) IgG3 (C) IgG4 (D) IgE above threshold, T, derived from the percentile (T) in the mean-normalised variant concentration distributions in the Control Cohort.

Article Snippet: IgG1 spike reference materials from Sinobiological (40590-D001) were used to calibrate the surface density of spike protein for each variant, binding to the conserved S2 region.

Techniques: Variant Assay, Derivative Assay, Concentration Assay, Control